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KMID : 0665220050180010039
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Korean Journal of Food and Nutrition
2005 Volume.18 No. 1 p.39 ~ p.47
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Medium Optimization for Fibrinolytic Enzyme Production by Bacillus subtilis MG410 Isolated
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Lee Joo-Yeon
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Abstract
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Using the bacteria isolated from Chungkookjang, Bacillus sublilis MG410 which is excellent in fibrinolytic enzyme activity was isolated. In increase the high production of fibrinolytic enzyme from Bacillus sublilis MG410, the effect of various carbon sources, nitrogen sources, inorganic sources, the initial pH of medium were investigated. The most effective carbon and nitrogen sources were founded cellobiose 0.5%(w/v) and soybean meal 2%(w/v) respectively. None of inorganic sources examined had any detectable stimulating effect on fibrinolytic enzyme production except Na₂HPO₄·12H₂O. The initial optimum pH for fibrinolytic enzyme production ranged from 5∼6 and agitation speed was effect at 150rpm. In jar fermentor experiments under optimal culture conditions, the activity of fibrinolytic enzyme reached about 5.050 unit after 48hours.
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KEYWORD
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Chungkookjang, Bacillus subtilis, fibrinolytic enzyme
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